{"componentChunkName":"component---src-templates-atlas-page-tsx","path":"/bbi/fly-chromatin-accessibility/","result":{"data":{"config":{"frontmatter":{"title":"Data Visualization"}},"page":{"html":"","frontmatter":{"title":"BBI Fly Chromatin Accessibility Atlas","description":"BBI Fly Chromatin Accessibility Atlas","keywords":"Fred Hutch Data Visualization Brotman Baty","background":"/img/bg-nygc.jpg","icon":"https://brotmanbaty.org/wp-content/uploads/2018/06/Logo-square.png","footer":"{ \"logo\": [ {\"src\":\"/img/logos/footer-gray-aicl.png\",\"href\":\"https:\\/\\/www.allen-lineage.org/\"}, {\"src\":\"/img/logos/footer-gray-bbi.png\",\"href\":\"https:\\/\\/brotmanbaty.org/\"}, {\"src\":\"/img/logos/footer-gray-uwgs.png\",\"href\":\"https:\\/\\/www.gs.washington.edu/\"} ], \"twitter\": \"https://twitter.com/FHDataViz\", \"linkedin\": \"https://www.linkedin.com/company/fhdataviz\", \"instagram\": \"https://www.instagram.com/fhdataviz/\" }","models":null,"transforms":null,"components":[{"component":"atlas-marquee","props":"{ \"title\": \"descartes\", \"subtitle\": \"A Chromatin Cell Atlas of the Developing Fly Embryo\", \"link\": \"/\" }"},{"component":"atlas-links","props":"{ \"title\": \"Chromatin accessibility in developing Drosophila melanogaster embryos at single-cell resolution\", \"text\": \"Here we investigate the dynamics of chromatin regulatory landscapes during embryogenesis at single-cell resolution. Using single-cell combinatorial indexing assay for transposase accessible chromatin with sequencing (sci-ATAC-seq)1, we profiled chromatin accessibility in over 20,000 single nuclei from fixed Drosophila melanogaster embryos spanning three landmark embryonic stages: 2–4 h after egg laying (predominantly stage 5 blastoderm nuclei), when each embryo comprises around 6,000 multipotent cells; 6–8 h after egg laying (predominantly stage 10–11), to capture a midpoint in embryonic development when major lineages in the mesoderm and ectoderm are specified; and 10–12 h after egg laying (predominantly stage 13), when each of the embryo’s more than 20,000 cells are undergoing terminal differentiation.\", \"links\": [{ \"name\": \"Publication\", \"url\": \"https://www.nature.com/articles/nature25981\" }, { \"name\": \"Tutorial\", \"url\": \"/bbi/fly-chromatin-accessibility/tutorial\" }, { \"name\": \"GitHub\", \"url\": \"https://github.com/shendurelab/fly-atac\" } ] }"},{"component":"atlas-carousel","props":"{ \"title\": \"Study Design\", \"imageSize\": [450, 270], \"data\": [ { \"title\": \"Refine cell clustering to identify cell-types within germ layers\", \"image\": \"/img/carousel/bbiFly/graphic1.jpg\", \"info\": \"Our analysis used the similarity of chromatin accessibility landscapes in individual cells to identify how cell types become epigenomically encoded in the developing fly embryo. At 6-8 hours after egg laying, we identified 18 cell clusters consistent with individual cell types. You can use the code we provide here to see how we clustered cells and explore how various features are distributed across these clusters.\" }, { \"title\": \"Compare chromatin of germ layers through differential accessibility\", \"image\": \"/img/carousel/bbiFly/graphic2.jpg\", \"info\": \"Having identified clusters of cells with distinct patterns of chromatin accessibility, an obvious next concern is which sites are open in one germ layer, but not the others. To address this question, we used a logistic regression framework to identify sites that were significantly more accessible in one germ layer. We present some of the code used to do that in this section of the tutorial.\" }, { \"title\": \"Order cells along developmental trajectories\", \"image\": \"/img/carousel/bbiFly/graphic3.jpg\", \"info\": \"Finally, we show in the paper that we can arrange these cells along a developmental trajectory in order to learn about the timing of individual regulatory elements opening and closing in development. For this analysis, we focused on the embryos from the earliest time point in our data set (2-4 hours after egg laying). The code for doing this kind of analysis is presented in the fourth section of the tutorial.\" } ] }"},{"component":"atlas-downloads","props":"{ \"title\": \"Download\", \"sections\":  [ { \"headline\":\"Here are links to all of the data used in the tutorial (code is presented for downloading and processing these files through the course of the tutorial too).\", \"desc\":\"\", \"files\":[ { \"name\":\"irlba_1.0.3.tar.gz\", \"desc\":\"Source code for irlba version 1.0.3\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/irlba_1.0.3.tar.gz\", \"size\":\"177K\", \"type\":\"GZ\", \"date\":\"18-Nov-2017\" }, { \"name\":\"monocle_2.3.5.tar.gz\", \"desc\":\"Source code for Monocle version 2.3.5\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/monocle_2.5.3.tar.gz\", \"size\":\"16M\", \"type\":\"GZ\", \"date\":\"18-Nov-2017\" }, { \"name\":\"DDRTree_0.1.4.tar.gz\", \"desc\":\"Source code for DDRTree version 0.1.4\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/monocle_2.5.3.tar.gz\", \"size\":\"12K\", \"type\":\"GZ\", \"date\":\"18-Nov-2017\" }, { \"name\":\"monocle_patch.R\", \"desc\":\"Differential Gene Test\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/monocle_patch.R\", \"size\":\"6.6K\", \"type\":\"R\", \"date\":\"18-Nov-2017\" }, { \"name\":\"6to8.2kbmatrix.sparse.binary.rds\", \"desc\":\"6 to 8 2kb matrix sparse binary\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.2kbmatrix.sparse.binary.rds\", \"size\":\"113M\", \"type\":\"RDS\", \"date\":\"18-Nov-2017\" }, { \"name\":\"6to8.summitmatrix.sparse.binary.rds\", \"desc\":\"6 to 8 summit matrix sparse binary\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.summitmatrix.sparse.binary.rds\", \"size\":\"69M\", \"type\":\"RDS\", \"date\":\"18-Nov-2017\" }, { \"name\":\"6to8.xycalls.txt\", \"desc\":\"Sex assignment, 6-8 hour time point\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.xycalls.txt\", \"size\":\"300K\", \"type\":\"TXT\", \"date\":\"18-Nov-2017\" }, { \"name\":\"6to8.read.report.txt\", \"desc\":\"Total number of unique reads\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.read.report.txt\", \"size\":\"429K\", \"type\":\"TXT\", \"date\":\"18-Nov-2017\" }, { \"name\":\"2to4_files.tar.gz\", \"desc\":\"Pseudotemporal ordering\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/2to4_files.tar.gz\", \"size\":\"44M\", \"type\":\"GZ\", \"date\":\"18-Nov-2017\" } ] }, { \"headline\":\"Accessibility Matrices\", \"desc\":\" Here we make peak of accessibility by cell matrices for all three time points. Each matrix is a sparse matrix file in .rds format. Requires the Matrix package in R to open.\", \"files\":[ { \"name\":\"2to4.summitmatrix.sparse.binary.rds\", \"desc\":\"Accessiblity matrix 2-4hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/2to4.summitmatrix.sparse.binary.rds\", \"size\":\"59M\", \"type\":\"RDS\", \"date\":\"8-Jun-2019\" }, { \"name\":\"2to4.summitmatrix.sparse.binary.rds\", \"desc\":\"Accessiblity matrix 6-8hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.summitmatrix.sparse.binary.rds\", \"size\":\"59M\", \"type\":\"RDS\",  \"date\":\"18-Nov-2017\" }, { \"name\":\"10to12.summitmatrix.sparse.binary.rds\", \"desc\":\"Accessiblity matrix 10-12hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/10to12.summitmatrix.sparse.binary.rds\", \"size\":\"48M\", \"type\":\"RDS\", \"date\":\"9-Jun-2019\" } ] }, { \"headline\":\"t-SNE Coordinates\", \"desc\":\"Here we make t-SNE coordinates for each timepoint available. See the manuscript for how t-SNE coordinates were calculated. Each file is a gzipped .tsv and includes 3 columns: Cell ID column, x-axis coordinate column, y-axis coordinate column.\", \"files\":[ { \"name\":\"2to4.tsnecoords.txt.gz\", \"desc\":\"t-SNE coordinates 2-4hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/2to4.tsnecoords.txt.gz\", \"size\":\"165K\", \"type\":\"GZ\", \"date\":\"9-Jun-2019\" }, { \"name\":\"6to8.tsnecoords.txt.gz\", \"desc\":\"t-SNE coordinates 6-8hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.tsnecoords.txt.gz\", \"size\":\"162K\", \"type\":\"GZ\", \"date\":\"9-Jun-2019\" }, { \"name\":\"10to12.tsnecoords.txt.gz\", \"desc\":\"t-SNE coordinates 10-12hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/10to12.tsnecoords.txt.gz\", \"size\":\"149K\", \"type\":\"GZ\", \"date\":\"9-Jun-2019\" } ] }, { \"headline\":\"Cluster Assignments\", \"desc\":\"Here we make the density peak-based cluster assignments (derived from the t-SNE coordinates) for each timepoint available. See the manuscript for how t-SNE coordinates were calculated and how density peak clusters were identified. Each file is a gzipped .tsv and includes 2 columns: Cell ID column, cluster assignment.\", \"files\":[ { \"name\":\"2to4.densitypeakclusters.txt.gz\", \"desc\":\"Cluster assignments 2-4hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/2to4.densitypeakclusters.txt.gz\", \"size\":\"34K\", \"type\":\"GZ\", \"date\":\"8-Jun-2019\" }, { \"name\":\"6to8.densitypeakclusters.txt.gz\", \"desc\":\"Cluster assignments 6-8hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/6to8.densitypeakclusters.txt.gz\", \"size\":\"34K\", \"type\":\"GZ\", \"date\":\"8-Jun-2019\" }, { \"name\":\"10to12.densitypeakclusters.txt.gz\", \"desc\":\"Cluster assignments 10-12hr timepoint\", \"url\":\"http://krishna.gs.washington.edu/content/members/cusanovich/fly_embryogenesis/updated_data/vignette/10to12.densitypeakclusters.txt.gz\", \"size\":\"31K\", \"type\":\"GZ\", \"date\":\"8-Jun-2019\" } ] } ] }"}]}}},"pageContext":{"id":"bce004fb-c4fc-5e96-8fbe-2c6b6c6d8b7e","cid":"436359cb-2391-57ee-8d31-d691fb5401b9"}},"staticQueryHashes":[]}